http://hdl.handle.net/123456789/103 2026-04-05T17:20:52Z 2026-04-05T17:20:52Z WAKAWA, Lucky Dartsa http://hdl.handle.net/123456789/2296 2024-05-23T11:59:18Z 2023-06-13T00:00:00Z

MORPHO-MOLECULAR CHARACTERISTICS AND PHYSICO-CHEMICAL PROPERTIES OF BALANITES AEGYPTIACA (L.) DELILE IN THE SAHELIAN ZONE OF NIGERIA WAKAWA, Lucky Dartsa Balanites aegyptiaca (BURKAN) is a wild fruit tree of high ethnomedicinal importance in the Sahelian zone of Nigeria for treating ailments like typhoid and malaria. The wild populations of the species are threatened by overexploitation and habitat loss. Variation in plant leaf, fruit morphology, genetic diversity, and physico-chemical properties from different locations which are essential for superior trait selection and vital to developing strategies for its domestication and conservation, is limited in Nigeria. This study was therefore conducted to determine the fruit and leaf morphology, physico-chemical and molecular characteristics of Balanites aegyptiaca in the Sahelian zone of Nigeria. Eight locations in five states: Baure and Mashi (Katsina), Buratai (Borno), Dumsai and Gashua (Yobe), Gamawa (Bauchi), Guri and Kirikasama (Jigawa) were purposively selected, based on the availability of Balanites aegyptiaca trees. Ten mature trees were randomly selected from each location. Thirty ripe fruits and leaves were randomly collected from each tree. Fruit Length (FL, cm), Fruit Weight (FW, g), Fruit Thickness (FT, cm), and Pulp Weight (PW, g) were determined. Leaf morphology: Leaf Length (LL, cm), Leaf Width (LW, cm), and Leaf Thickness (LT, cm) were measured. Oil was extracted from the fruit kernels obtained from each location using soxhlet extraction method. Physico-chemical properties of extracted oil: refractive index, viscosity (cP), acid, and iodine values (mgKOH/g) were analysed. Genetic characteristics of selected trees were determined using chloroplast gene sequences of matK region. Nucleotide diversity (Pi), Parsimony informatics sites (Ps), Polymorphic sites (S), and average number of nucleotide difference (k) were determined following standard procedures. Data were analysed using descriptive statistics, cluster analysis, and ANOVA at α0.05. The FL varied significantly from 2.45±0.31 (Dumsai) to 3.08±0.26 (Kirikasama), while FW ranged from 1.77±0.19 (Gashua) to 2.13±0.16 (Baure). Baure had the highest FT (1.84±0.39), while Guri had the least, (1.41±0.15). The PW significantly decreased from 4.44±1.87 (Gamawa) to 2.18±1.33 (Buratai). The LL, LW, and LT significantly increased from 0.41±0.05 (Dumsai) to 0.62±0.35 (Baure); 0.21±0.13 (Buratai) to 0.44±0.19 (Baure); and 0.41±0.05 (Dumsai) to 0.62±0.35 (Baure), respectively. This supported the feasibility of location as a criterion for selection in trait improvement. Refractive index and viscosity increased from 1.36±0.15 (Mashi) to 1.48±0.06 (Gamawa) and 41.33±2.08 (Gashua) to 48.67±2.52 (Buratai), respectively. Acid and iodine values varied significantly from 1.36±0.07 (Dumsai) to 2.11±0.07 (Baure) and 67.07±1.53 (Gamawa) to 85.33±2.52 (Baure), respectively. The Pi and Ps varied from 0.002 (Dumsai) to 0.264 (Mashi) and 0.00 (Dumsai) to 2.00 (Mashi), respectively. The S and k ranged from 3.0 (Dumsai) to 302.00 (Mashi) and 2.00 (Dumsai) to 151.70 (Mashi), respectively. The high genetic diversity in Mashi signified germplasm potential for species improvement. Species population in Baure and Mashi formed a distinct cluster with the highest bootstrap value (100), while the other populations formed a single cluster with bootstrap value of 4. The genetically diverse population in Mashi is a potential source for superior germplasm required for the domestication and improvement of Balanites aegyptiaca and could play vital roles in germplasm collection.

2023-06-13T00:00:00Z ADENAIYA, Adewunmi Omobolaji http://hdl.handle.net/123456789/1792 2024-04-18T14:52:43Z 2021-10-01T00:00:00Z

BIOINCISION OF Gmelina arborea Roxb. HEARTWOOD FOR PERMEABILITY IMPROVEMENT TO CHEMICAL TREATMENT ADENAIYA, Adewunmi Omobolaji Gmelina arborea is a versatile but moderately refractory timber species whose heartwood is impermeable to chemical treatment due to tyloses deposition which occlude its vessels. This makes its utilisation unsustainable for a broad range of technical applications. Bioincision is an emerging procedure capable of improving the permeability of wood to fluid treatment, but information on its suitability in improving the permeability of Gmelina arborea heartwood is limited. Therefore, the effect of bioincision on permeability, anatomical and mechanical properties of Gmelina arborea heartwood was investigated. Five 34-year-old Gmelina arborea trees were harvested at the University of Ibadan plantation. A bolt of 300 cm was obtained from the base of each tree. Heartwood of each bolt was extracted and converted into standard dimensions. Wood samples (n=100) were bioincised with two white-rot fungi: Inonotus dryophilus (ID, 999) and Ganoderma adspersum (GA, CBS109416) for 9 weeks, while unincised samples (n=50) served as control. Ten samples (20 mm x 20 mm x 60 mm) each from bioincised and unincised treatments were pressure-impregnated for 90 minutes with Tanalith (5.5% concentration) for permeability tests. Thereafter, samples from bioincised and unincised (n=30) were pressure-treated with liquid dye and depth of penetration was evaluated. Ten samples (10 mm x 10 mm x 10 mm) each were obtained from bioincised treatments to assess effect of fungi on wood anatomy. Tanalith absorption (Kg/m3) and retention (Kg/m3) were calculated. Axial Penetration Depth (APD, mm); Axial Penetration Area (APA, %); Tangential Penetration Depth (TPD, mm); Radial Penetration Depth (RPD, mm); Lateral Penetration Area (LPA, %) were measured. Modulus of Elasticity (MOE, MPa); Modulus of Rupture (MOR, MPa); Maximum Compressive Strength (MCS//, N/mm2); Janka Hardness (JH, N) of unincised and bioincised samples were determined using standard methods. Data obtained were analysed using descriptive statistics and ANOVA at α0.05. Unincised samples had least absorption (112.0±8.5) and retention (6.2±0.5), while GA incised samples had highest (135.0±16.6 and 7.4±0.9), respectively. The APD significantly varied from 7.0±1.7 (unincised samples) to 31.2±4.1 (GA incised samples), implying higher degradation of vessel tyloses by GA. The APA increased from 0.07±0.03 (unincised samples) to 3.1±1.9 (ID incised samples). The TPD ranged from 0.4±0.2 (unincised samples) to 3.0±2.0 (GA incised samples), indicating increased lateral penetration. The RPD were 0.1±0.1 and 4.8±2.2 in unincised and GA incised samples, respectively. The LPA varied significantly from 0.001±0.001 (unincised samples) to 17.0±9.4 (GA incised samples). Both fungi induced delamellation of axial parenchyma cells and degraded tyloses within the wood vessels. Fungal hyphae of GA and ID were predominantly found in vessels and ray parenchyma cells. The MOE ranged from 7305.0±298.1 (GA incised) to 7771.1±256.0 (unincised samples). The MOR varied significantly from 70.8±6.1 to 77.9±3.9 in GA incised samples and unincised samples, respectively. The MCS// were 42.2±1.8 and 43.1±1.4 in GA incised samples and unincised samples, respectively. The JH increased from 2241.0±115.5 (unincised samples) to 2593.1±208.7 (GA incised samples). Bioincising Gmelina arborea heartwood with Ganoderma adspersum effectively dissolved occlusions within the vessels. Wood bioincision improved permeability, with negligible effects on the mechanical properties.

2021-10-01T00:00:00Z ONEFELI, Alfred Ossai http://hdl.handle.net/123456789/1600 2022-03-02T11:21:14Z 2021-08-01T00:00:00Z

MORPHOLOGIC AND MOLECULAR CHARACTERISATION OF Ricinodendron heudelotii (Baill.) Pierre ex Pax IN SOUTHERN NIGERIA ONEFELI, Alfred Ossai Ricinodendron heudelotii is an ethnomedicinally important indigenous tree species found in wild populations in Nigeria, but its utilisation is limited by insufficient taxonomic information. Morphologic and genetic characterisations provide detailed taxonomic description for effective identification of indigenous tree species. However, such information is scarce with respect to Ricinodendron heudelotii in Southern Nigeria. Therefore, morphology and molecular characteristics of leaf and fruit of Ricinodendron heudelotii trees in Southern Nigeria were investigated. Wild Ricinodendron heudelotii trees were purposively selected from Oyo [Ibadan (n=1) and Onigambari (n=5)], Ondo [Oloruntele (n=12) and Akure (n=4)], Osun [Osu (n=8), Ikoyi (n=4) and Ile-Ife (n=10)], Edo [Benin (n=4)] and Cross River [Boki (n=2)] States, based on availability. Fifty random samples of uniformly sized leaves, from each location, were assessed for Leaf Length (LL, cm), Petiole Length (PL, cm), epidermal cell shape, Guard Cell Area (GCA, µm2), Pore Size (PS, µm2), Stomata Length (SL, µm) and epidermal cell length (ECL, µm) following standard methods. For fruit and seed morphology, 50 matured fruits were randomly collected from each location and used to determine Fruit Length (FL, mm), Pulp Weight (PW, g), Fruit Largest Width (FLW, mm), Fruit Roundness Ratio (FRR), seed length (mm) and Seed Diameter (SD, mm2) using standard procedures. Six leaves from each location were subjected to molecular characterisation using 19 Inter Simple Sequence Repeat (ISSR) markers following standard methods. Polymorphic Information Content (PIC), genetic diversity, similarity index, and unique allele were determined. Data were analysed using descriptive statistics, Principal Component Analysis (PCA), Cluster Analysis and ANOVA at α0.05. Leaf length significantly increased from 22.3±5.7 (Osu) to 53.0±5.8 (Onigambari), while PL varied from 8.9±0.1 (Boki) to 30.9±5.0 (Onigambari). Epidermal cells were polygonal in all sites, except Akure with irregular shape. Highest GCA (243.1±30.5), PS (322.8±78.5), SL (29.4±2.4) and ECL (43.7±8.8) were in Akure, while the least were in Ikoyi (72.7±7.0), Onigambari (40.3±8.0), Ikoyi (20.4±3.6) and Ibadan (19.2±8.7), respectively. Boki had highest (45.4±2.6) FL, while Ile-Ife had least (30.2±11.5). The PW and FLW were highest at Oloruntele (34.3±7.2; 44.2±4.0) and least at Akure (18.4±3.3; 31.2±1.3). The FRR and seed length varied from Oloruntele (14.4±1.7; 0.77±0.3) to Akure (17.1±0.7; 1.31±0.11), while SD ranged from 12.9±0.9 (Akure) to 16.3±0.6 (Ile-Ife). The GCA (0.48), PS (0.57) and SL (0.39) had highest contribution to the 76.8% total variance in leaf and fruit morphometrics. The ISSR marker-840 had highest PIC (0.42), while ISSR marker-848 had the least (0.21). Genetic diversity increased from Akure (0.09) to Oloruntele (0.24). Highest genetic similarity (60.3%) was between Ibadan and Osu, while the least (1.0%) was between Akure and Benin. Three unique allele (1600-2000bp) were identified in Oloruntele. The population of Ricinodendron heudelotii clustered into four groups; Akure, Benin and Oloruntele were distinct, while others formed a group. Leaf-based characters showed distinct taxonomic differences across populations of Ricinodendron heudelotii in Southern Nigeria. The most genetically diverse population was found in Oloruntele, which indicates potential germplasm for domestication of the species. The unique alleles identified could be used for marker assisted identification of the population.

2021-08-01T00:00:00Z JEGEDE, Opeyemi Christianah http://hdl.handle.net/123456789/1279 2022-02-18T10:03:17Z 2020-01-01T00:00:00Z

REPRODUCTIVE PHENOLOGY AND MOLECULAR CHARACTERISATION OF Moringa oleifera LAM. LANDRACES IN RAIN FOREST ZONE OF SOUTHWESTERN NIGERIA JEGEDE, Opeyemi Christianah Local adaptation of multipurpose tree species such as Moringa oleifera has resulted in the emergence of landraces, which could influence selection for mass propagation. It has been established that knowledge on landraces affects decisions on germplasm collection for propagation of Moringa oleifera. However, critical information on the reproduction and genetic characteristics of different landraces are limited. Therefore, the flowering and fruiting patterns, pod morphology, seedling growth and genetic characteristics of Moringa oleifera in Southwestern Nigeria were investigated. Two Moringa plantations were purposively selected from each of eight locations: Abeokuta, Akure, Erinjiyan, Ijare, Ijari, Ijaye, Omu, and Oyo, based on availability. Five trees were randomly selected from four corners and the centre of a 20m by 20m plot, demarcated at the centre of each plantation. These trees were used to assess phenology: onset and duration of flowering and fruiting (days), pod morphology and maturity index [duration before pod colour change (days)], for 24 months. Pods (300) were collected from each location and measured for length (cm), diameter (mm), seed weight (g) and number of seed/pod (NS). Seeds extracted at each stage of maturity were subjected to germination test using standard procedures. Sixty uniformly growing seedlings were selected per location and monitored for height (cm), collar diameter (mm) and number of leaves for six months. Genetic characteristics of five accessions/plantation (n=80) were determined using five microsatellite markers (MO8, MO15, MO48, MO61, MO64). Number of Alleles (NA), allele frequency, genetic diversity and Polymorphic Information Content (PIC) were determined. Data were analysed using descriptive statistics, Analysis of Molecular Variance (AMOVA), Cluster Analysis and ANOVA at α0.05. Flowering (April-June; August-October) and fruiting (June-September; October-February) occurred twice a year; while duration of flowering (43.5-44.3) and fruiting (154-160) in days were similar across locations. Three stages of pod colour change: green (26.5-28.8), yellow (82.6-92.3) and brown (25.8-31.8) were observed. Abeokuta (40.0±1.7) had the highest pod length, while Erinjiyan (27.6±0.6) had the least. Pods from Akure (16.4±3.2) had the least diameter while Abeokuta (19.5±2.9) had highest. Seed weight was significantly highest (31.4±1.7) at Omu and least (17.2±1.0) at Ijari, while NS ranged from 12.9±3.3 (Erinjiyan) to 17.9±3.3 (Akure). Seed germination was highest (90.7±0.3%) for yellow pods and least (30.7±0.9%) for green. Height was significantly highest (112.7±1.4) for seedlings from Ijari and least (76.9±0.8) for those from Omu. Seedling collar diameter ranged from 21.7±0.6 (Akure) to 37.4±0.9 (Erinjiyan). Ninety-six alleles with an average of 9.6±0.6 alleles/locus in each accession were amplified. Allele frequency and gene diversity ranged from 0.2% to 0.5% and 0.7% to 0.9%, respectively. The highest PIC (0.9) occurred in MO64, while MO48 had the least (0.6). There were significant differences in intra-specific (48%) and inter-specific (52%) genetic diversity of the sampled populations. Five clusters were identified with similarity coefficients that ranged from 0.1 to 0.4. Moringa oleifera population in Southwestern Nigeria exhibited extensive and simultaneous flowering and fruiting patterns. Pod and seed morphology were location dependent and influenced seedling growth. Five landraces of Moringa oleifera were identified and this has implications in germplasm selection for propagation

2020-01-01T00:00:00Z