dc.description.abstract |
Polycystic Ovary Syndrome (PCOS) is an endocrine disorder with a global prevalence of 5-10%
among women of reproductive age. Women with PCOS have a 2.7-fold increased risk of developing
ovarian and cervical cancers. Ethnobotanical survey revealed that plants including Kigelia africana
(Lam.) Benth. (KA), Basella alba L. (BA), Tetracera potatoria G. Don (TP) and Mormodica
charantia L. (MC) are used for treatment of PCOS in southwestern Nigeria. However, there is no
reported scientific evidence to validate these claims. This study was, therefore, designed to
investigate the effect of the four plants in alleviating polycystic ovary conditions in rats and the
associated risk of ovarian and cervical cancers.
Letrozole (1 mg/kg) was used for the induction of PCOS in thirty female albino rats (180-200 g,
n=5). Methanol leaf extracts of KA, BA, TP and MC (100 mg/kg b.w.) and Clomiphene citrate (1
mg/kg b.w., standard drug) were administered for 15 days. Histopathological evaluation of the
ovaries was done microscopically. Luteinizing hormone (LH), follicle stimulating hormone (FSH)
and estradiol were measured using ELISA. Extracts of the most active plants, KA (FHI-111350) and
TP (IFE-17794) were successively partitioned into n-hexane, dichloromethane and ethyl acetate
fractions, and screened for PCOS inhibitory activity. Compounds were isolated from the active
fractions using chromatographic techniques. Structures of isolated compounds were elucidated using
spectroscopic techniques. Anti-proliferative effect of fractions, isolated compounds and derivatised
cinnamic acid analogues were determined on cervix adenocarcinoma (HeLa) and Chinese Hamster
ovarian (CHO 1) cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
(MTT) assay. Doxorubicin was used as standard. Data were analysed using one-way ANOVA
followed by Student’s t-test at α0.05.
Animals treated with KA showed normal ovarian stroma with moderate fibroblastic tissues. The
levels of FSH in KA, BA, TP and MC treated groups were 0.96±0.08, 1.10±0.23, 0.81±0.04 and
0.69±0.01 mIU/mL, respectively compared to control group (0.93±0.19 mIU/mL). The TP
(0.19±0.05 mIU/mL) significantly reduced the level of LH compared with the control group
(0.22±0.0l mIU/mL). Hexane and ethyl acetate fractions displayed selective moderate inhibitory
effect (IC50 = 5.3±1.10 µg/mL) on CHO 1 cell line compared to doxorubicin (IC50 = 0.8±0.01 µg/mL).
Compounds 3-(3, 4-dimethoxyphenyl) acrylic acid (1), sitosterol (2), methyl 3-(3,4-
dihydroxyphenyl) acrylate (3), 2, 3-dihydro-5-(hydroxymethyl) furan-2, 3, 4-triol (4), p-coumaric
acid (5) and caffeic acid (6) were isolated from KA, while apigenin (7) was isolated from TP.
iii
Compound 1 displayed moderate anti-proliferative activity against HeLa cell line (IC50 = 33.5±0.60
µg/mL). Compound 7 inhibited proliferation of HeLa cell line (IC50 = 6.2 µg/mL) and had moderate
inhibitory effect on CHO l cell line (IC50 = 22.2 µg/mL). Derivatised compound N'-(2, 6-
dimethoxybenzylidene)-3-(4-methoxyphenyl) acrylohydrazide (8) inhibited both CHO l (IC50 =
l8.4±4.l µg/mL) and HeLa (IC50 = 22.4±0.4 µg/mL) cells.
The extracts of Kigelia africana and Tetracera potatoria had inhibitory effects on polycystic ovary
condition, irregular oestrual cycle and hormonal imbalance in female albino rats. The
phenylpropanoids and derivatised analogues exhibited antiproliferative effect on ovarian and cervical
cancer cell lines, which could contribute to their use for management of polycystic ovary syndrome. |
en_US |